Tan A. Ince, MD, PhD - Play for P.I.N.K.

Scientific Director, Live Tumor Culture Core and Tissue Bank Core Facility
Director, Tumor Stem Cell Division,
Interdisciplinary Stem Cell Institute
Associate Professor, Department of Pathology
University of Miami
Miami, Florida

Areas of Focus: Treatment and Tumor Biology

2018-2019 PROJECT

The Play for P.I.N.K. Award in honor of Laura Lassman and in memory of Nicholas Lassman

Current Research:

Seeking novel approaches for the treatment of breast cancer by targeting cancer stem cells.
Laboratory studies are conducted to test a drug cocktail to target multiple regulators of cancer stem cell growth.
If successful, these studies could reveal a non-chemotherapeutic breast cancer stem cell targeted therapy.

Hormones play key roles in cell growth, including the growth of breast cancer cells. Dr. Ince’s group has shown that hormones known to promote breast cancer by stimulating cell growth can also promote cancer by altering the DNA in the cancer cells. He is conducting studies to understand how this happens to identify new treatment and prevention strategies.

Full Research Summary:

Many environmental and lifestyle factors including diet, exercise, and hormones influence development of breast cancer. While lifestyle or hormonal factors do not cause genetic mutations outright, they may affect gene regulation in a non-genetic way.

Dr. Ince’s laboratory discovered that estrogen receptors, androgen receptors, and vitamin D receptors work with DNA packaging protein in a so called “epigenetic program” (attaching reversible chemical modification of the DNA) to regulate cancer stem cells.

The group previously reported that patients with breast cancers that express estrogen receptors, androgen receptors, and vitamin D receptors are seven-fold more likely to survive compared to patients with breast cancer that expresses none of these receptors.

While exploring the potential mechanism of this hormonal regulation, they discovered that hormonal signals are converted into heritable information that can be passed on the daughter cells without changing DNA sequence.

In the coming year, Dr Ince’s team will study how these hormones change DNA structure with the goal of developing new strategies for treatment.

This work can potentially explain how hormones and diet influence breast cancer.

BIO

Dr. Ince received his PhD in Pharmacology from Cornell and completed clinical training at Massachusetts General Hospital, Brigham and Women’s Hospital, and Harvard Medical School. Dr. Ince was a visiting clinical scientist at Massachusetts Institute of Technology, 2000-2007, where he developed a new cell culture nutrient medium that is now widely used to grow human breast and ovary cells. In 2010, he was recruited to the Braman Family Breast Cancer Institute at the University of Miami Miller School of Medicine.

Dr. Ince’s research focuses on the role of normal cell-of-origin in determining tumor phenotype and development of culture systems for in vitro culture of primary human tissues and tumors. The use of normal cell types as a reference to classify tumors, however, has not been widely emulated in solid tumors, partly due to a more limited understanding of epithelial cell differentiation. Dr. Ince used a new and innovative multiplexed immunofluorescence technology to monitor multiple markers simultaneously in the very same cells. Based on the study, Dr. Ince and colleagues described eleven new normal breast subtypes and showed that each human tumor is similar to one of these normal cell types. These results led to new normal cell type-based classification of breast cancers that has important implications for understanding breast cancer prognostics and how breast cancer is treated. In brief, the results of this study indicate that breast tumors arising from different normal cells have significantly different clinical outcomes and drug response, and should be classified and treated differently.